PCR

Pt.1. Basic principles and methods

What is PCR?

Instrumentation, reagents and consumables

Amplifying the correct product

Pt.2. Techniques and applications

Cloning and modification of PCR products

Isolation and construction of DNA clones

PCR mutagenesis

Sequencing PCR products

New sequence determination and genome mapping

Figerprinting

Characterizing unknown mutations

Analysis of known mutations

Detecting pathogens

Quantitative PCR

Appendices

Index.